|
|
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result sql = [Use of a microfluorometric method for measuring free calcium in the cytoplasm of isolated cultured rat cardiomyocytes].
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result sql = Dual wavelength microfluorometry was used to measure the cytoplasmic free calcium concentration [( Ca2+]in) in single cultured cells from ventricular myocytes of neonatal rats loaded with the indicator fura-2. At 2.5 nmol/l extracellular Ca2+ in the resting cells [Ca2+]in was between 80 and 110 nmol/l. Sometimes, spontaneous low-frequency (approximately 0.1 Hz) [Ca2+]in oscillations were observed. High-potassium depolarization led to a Ca2+-antagonists-sensitive rise of [Ca2+]in. Both caffeine++ (5-10 mmol/l) and thymol (lmmol/l) initialized transient increase of [Ca2+]in. Mechanisms of [Ca2+]in homeostasis in heart muscle cells were discussed.
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char = o;code = 111
char = f;code = 102
char = ;code = 32
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char = ;code = 32
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = f;code = 102
char = l;code = 108
char = u;code = 117
char = o;code = 111
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char = o;code = 111
char = m;code = 109
char = e;code = 101
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = c;code = 99
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char = e;code = 101
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char = o;code = 111
char = d;code = 100
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char = o;code = 111
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char = d;code = 100
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char = c;code = 99
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char = l;code = 108
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char = e;code = 101
char = s;code = 115
char = ];code = 93
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result sql = [Use of a microfluorometric method for measuring free calcium in the cytoplasm of isolated cultured rat cardiomyocytes].
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result sql = Dual wavelength microfluorometry was used to measure the cytoplasmic free calcium concentration [( Ca2+]in) in single cultured cells from ventricular myocytes of neonatal rats loaded with the indicator fura-2. At 2.5 nmol/l extracellular Ca2+ in the resting cells [Ca2+]in was between 80 and 110 nmol/l. Sometimes, spontaneous low-frequency (approximately 0.1 Hz) [Ca2+]in oscillations were observed. High-potassium depolarization led to a Ca2+-antagonists-sensitive rise of [Ca2+]in. Both caffeine++ (5-10 mmol/l) and thymol (lmmol/l) initialized transient increase of [Ca2+]in. Mechanisms of [Ca2+]in homeostasis in heart muscle cells were discussed.
Publication: Fiziol Zh 1989 Nov-Dec ;35(6)::39-45.
Publication Date: 1989 Nov-Dec
Study Author(s): Belan, P V;Verkhratskiĭ, A N;Pronchuk, N F;Tepikin, A V;
Institution:
Shortcut link to this study:
Publication Date: 1989 Nov-Dec
Study Author(s): Belan, P V;Verkhratskiĭ, A N;Pronchuk, N F;Tepikin, A V;
Institution:
Shortcut link to this study:
Dual wavelength microfluorometry was used to measure the cytoplasmic free calcium concentration [( Ca2+]in) in single cultured cells from ventricular myocytes of neonatal rats loaded with the indicator fura-2. At 2.5 nmol/l extracellular Ca2+ in the resting cells [Ca2+]in was between 80 and 110 nmol/l. Sometimes, spontaneous low-frequency (approximately 0.1 Hz) [Ca2+]in oscillations were observed. High-potassium depolarization led to a Ca2+-antagonists-sensitive rise of [Ca2+]in. Both caffeine++ (5-10 mmol/l) and thymol (lmmol/l) initialized transient increase of [Ca2+]in. Mechanisms of [Ca2+]in homeostasis in heart muscle cells were discussed.
PMID: 2612622
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