|
|
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char = F;code = 70
char = l;code = 108
char = u;code = 117
char = o;code = 111
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = e;code = 101
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char = r;code = 114
char = i;code = 105
char = c;code = 99
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char = e;code = 101
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char = i;code = 105
char = n;code = 110
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char = o;code = 111
char = n;code = 110
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char = o;code = 111
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char = ;code = 32
char = s;code = 115
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char = l;code = 108
char = f;code = 102
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = l;code = 108
char = a;code = 97
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char = e;code = 101
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char = d;code = 100
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char = l;code = 108
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char = d;code = 100
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char = s;code = 115
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char = n;code = 110
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char = a;code = 97
char = n;code = 110
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char = m;code = 109
char = a;code = 97
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char = s;code = 115
char = u;code = 117
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char = s;code = 115
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char = w;code = 119
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char = t;code = 116
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char = m;code = 109
char = a;code = 97
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char = c;code = 99
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char = i;code = 105
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char = o;code = 111
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char = r;code = 114
char = a;code = 97
char = p;code = 112
char = h;code = 104
char = y;code = 121
char = ];code = 93
char = .;code = 46
result sql = [Fluorometric determination of sulfanilamide and sulfaguanidine residues in animal tissues with high-performance-liquid chromatography].
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char = i;code = 105
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char = n;code = 110
char = c;code = 99
char = e;code = 101
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char = u;code = 117
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char = m;code = 109
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char = (;code = 40
char = S;code = 83
char = A;code = 65
char = );code = 41
char = ;code = 32
char = a;code = 97
char = n;code = 110
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char = s;code = 115
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char = l;code = 108
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char = a;code = 97
char = g;code = 103
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char = a;code = 97
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char = d;code = 100
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = S;code = 83
char = G;code = 71
char = );code = 41
char = ;code = 32
char = r;code = 114
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char = i;code = 105
char = d;code = 100
char = u;code = 117
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
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char = a;code = 97
char = n;code = 110
char = i;code = 105
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = t;code = 116
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char = s;code = 115
char = s;code = 115
char = u;code = 117
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = m;code = 109
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char = g;code = 103
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char = a;code = 97
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char = ;code = 32
char = m;code = 109
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char = l;code = 108
char = k;code = 107
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char = a;code = 97
char = t;code = 116
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 1;code = 49
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char = m;code = 109
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char = l;code = 108
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char = ;code = 32
char = A;code = 65
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char = 2;code = 50
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char = o;code = 111
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char = 9;code = 57
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char = a;code = 97
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char = r;code = 114
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char = l;code = 108
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char = a;code = 97
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char = H;code = 72
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char = 8;code = 56
char = .;code = 46
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char = w;code = 119
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char = l;code = 108
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char = a;code = 97
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char = u;code = 117
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char = a;code = 97
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = d;code = 100
char = i;code = 105
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char = m;code = 109
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char = c;code = 99
char = a;code = 97
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char = e;code = 101
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char = ;code = 32
char = b;code = 98
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char = d;code = 100
char = d;code = 100
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char = A;code = 65
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char = c;code = 99
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char = f;code = 102
char = u;code = 117
char = g;code = 103
char = a;code = 97
char = t;code = 116
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char = o;code = 111
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char = a;code = 97
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char = a;code = 97
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char = a;code = 97
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
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char = s;code = 115
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char = a;code = 97
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char = N;code = 78
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result sql = A high-performance liquid chromatographic method is presented for the selective analysis of sulphanilamide (SA) and sulphaguanidine (SG) residues in animal tissues (muscle, liver), egg and milk at the 0.01-0.1 mg/kg level. A 25-g amount of the samples was extracted with 90 ml acetonitrile at pH 8.5 to which 2 ml saturated sodium carbonate had been added. After centrifugation, co-extracted water was separated by saturating the extract with sodium chloride and adding 50 ml dichloromethane. The residue of the dried and evaporated organic layer was partitioned between methanol, n-hexane and mobile phase (1 ml each). A 20-microliter volume of the aqueous layer was injected onto a Nucleosil 5 SA column [mobile phase 0.05 M sodium dihydrogenphosphate (adjusted to pH 2.0 with 85% orthophosphoric acid)-methanol, 85:15, v/v]. SA and SG were detected by their native fluorescence (excitation 275 nm, emission 340 nm) at retention times of 7 and 13 min, respectively, without interference of co-extractives. Parallel detection by ultraviolet absorption (275 nm) allowed on-line confirmation of the results. Recovery of spiked samples (0.1 mg/kg) was 80% (coefficient of variation, C.V. = 5%) for SA and 62% (C.V. = 6%) for SG. Concentrations of 0.27 mg/kg for SA and 0.05 mg/kg for SG were analyzed in muscle meat of a swine which received a single intramuscular injection of 2.1 g SA and 0.6 g SG and was slaughtered 24 h later.
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char = m;code = 109
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char = n;code = 110
char = c;code = 99
char = e;code = 101
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char = d;code = 100
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result sql = [Fluorometric determination of sulfanilamide and sulfaguanidine residues in animal tissues with high-performance-liquid chromatography].
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char = l;code = 108
char = p;code = 112
char = h;code = 104
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = l;code = 108
char = a;code = 97
char = m;code = 109
char = i;code = 105
char = d;code = 100
char = e;code = 101
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char = S;code = 83
char = A;code = 65
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char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = s;code = 115
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char = l;code = 108
char = p;code = 112
char = h;code = 104
char = a;code = 97
char = g;code = 103
char = u;code = 117
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = d;code = 100
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = S;code = 83
char = G;code = 71
char = );code = 41
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = i;code = 105
char = d;code = 100
char = u;code = 117
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = t;code = 116
char = i;code = 105
char = s;code = 115
char = s;code = 115
char = u;code = 117
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = m;code = 109
char = u;code = 117
char = s;code = 115
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = ,;code = 44
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = e;code = 101
char = g;code = 103
char = g;code = 103
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = l;code = 108
char = k;code = 107
char = ;code = 32
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 1;code = 49
char = -;code = 45
char = 0;code = 48
char = .;code = 46
char = 1;code = 49
char = ;code = 32
char = m;code = 109
char = g;code = 103
char = /;code = 47
char = k;code = 107
char = g;code = 103
char = ;code = 32
char = l;code = 108
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = .;code = 46
char = ;code = 32
char = A;code = 65
char = ;code = 32
char = 2;code = 50
char = 5;code = 53
char = -;code = 45
char = g;code = 103
char = ;code = 32
char = a;code = 97
char = m;code = 109
char = o;code = 111
char = u;code = 117
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = a;code = 97
char = m;code = 109
char = p;code = 112
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = 9;code = 57
char = 0;code = 48
char = ;code = 32
char = m;code = 109
char = l;code = 108
char = ;code = 32
char = a;code = 97
char = c;code = 99
char = e;code = 101
char = t;code = 116
char = o;code = 111
char = n;code = 110
char = i;code = 105
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = p;code = 112
char = H;code = 72
char = ;code = 32
char = 8;code = 56
char = .;code = 46
char = 5;code = 53
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = w;code = 119
char = h;code = 104
char = i;code = 105
char = c;code = 99
char = h;code = 104
char = ;code = 32
char = 2;code = 50
char = ;code = 32
char = m;code = 109
char = l;code = 108
char = ;code = 32
char = s;code = 115
char = a;code = 97
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = d;code = 100
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = r;code = 114
char = b;code = 98
char = o;code = 111
char = n;code = 110
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = h;code = 104
char = a;code = 97
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = e;code = 101
char = n;code = 110
char = ;code = 32
char = a;code = 97
char = d;code = 100
char = d;code = 100
char = e;code = 101
char = d;code = 100
char = .;code = 46
char = ;code = 32
char = A;code = 65
char = f;code = 102
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = f;code = 102
char = u;code = 117
char = g;code = 103
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = -;code = 45
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = p;code = 112
char = a;code = 97
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = s;code = 115
char = a;code = 97
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = d;code = 100
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
char = c;code = 99
char = h;code = 104
char = l;code = 108
char = o;code = 111
char = r;code = 114
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = a;code = 97
char = d;code = 100
char = d;code = 100
char = i;code = 105
char = n;code = 110
char = g;code = 103
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char = 5;code = 53
char = 0;code = 48
char = ;code = 32
char = m;code = 109
char = l;code = 108
char = ;code = 32
char = d;code = 100
char = i;code = 105
char = c;code = 99
char = h;code = 104
char = l;code = 108
char = o;code = 111
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = e;code = 101
char = t;code = 116
char = h;code = 104
char = a;code = 97
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char = e;code = 101
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = r;code = 114
char = e;code = 101
char = s;code = 115
char = i;code = 105
char = d;code = 100
char = u;code = 117
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = d;code = 100
char = r;code = 114
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char = e;code = 101
char = d;code = 100
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char = a;code = 97
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char = v;code = 118
char = a;code = 97
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char = a;code = 97
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = o;code = 111
char = r;code = 114
char = g;code = 103
char = a;code = 97
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char = i;code = 105
char = c;code = 99
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char = l;code = 108
char = a;code = 97
char = y;code = 121
char = e;code = 101
char = r;code = 114
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char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = p;code = 112
char = a;code = 97
char = r;code = 114
char = t;code = 116
char = i;code = 105
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = t;code = 116
char = w;code = 119
char = e;code = 101
char = e;code = 101
char = n;code = 110
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char = m;code = 109
char = e;code = 101
char = t;code = 116
char = h;code = 104
char = a;code = 97
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char = o;code = 111
char = l;code = 108
char = ,;code = 44
char = ;code = 32
char = n;code = 110
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char = h;code = 104
char = e;code = 101
char = x;code = 120
char = a;code = 97
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char = e;code = 101
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = m;code = 109
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char = b;code = 98
char = i;code = 105
char = l;code = 108
char = e;code = 101
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char = p;code = 112
char = h;code = 104
char = a;code = 97
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char = e;code = 101
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char = (;code = 40
char = 1;code = 49
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char = m;code = 109
char = l;code = 108
char = ;code = 32
char = e;code = 101
char = a;code = 97
char = c;code = 99
char = h;code = 104
char = );code = 41
char = .;code = 46
char = ;code = 32
char = A;code = 65
char = ;code = 32
char = 2;code = 50
char = 0;code = 48
char = -;code = 45
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = v;code = 118
char = o;code = 111
char = l;code = 108
char = u;code = 117
char = m;code = 109
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = a;code = 97
char = q;code = 113
char = u;code = 117
char = e;code = 101
char = o;code = 111
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = l;code = 108
char = a;code = 97
char = y;code = 121
char = e;code = 101
char = r;code = 114
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char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = j;code = 106
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = o;code = 111
char = n;code = 110
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = N;code = 78
char = u;code = 117
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = o;code = 111
char = s;code = 115
char = i;code = 105
char = l;code = 108
char = ;code = 32
char = 5;code = 53
char = ;code = 32
char = S;code = 83
char = A;code = 65
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = l;code = 108
char = u;code = 117
char = m;code = 109
char = n;code = 110
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char = [;code = 91
char = m;code = 109
char = o;code = 111
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = e;code = 101
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char = p;code = 112
char = h;code = 104
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 5;code = 53
char = ;code = 32
char = M;code = 77
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = d;code = 100
char = i;code = 105
char = u;code = 117
char = m;code = 109
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char = i;code = 105
char = h;code = 104
char = y;code = 121
char = d;code = 100
char = r;code = 114
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char = e;code = 101
char = n;code = 110
char = p;code = 112
char = h;code = 104
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char = s;code = 115
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char = h;code = 104
char = a;code = 97
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char = e;code = 101
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char = j;code = 106
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char = s;code = 115
char = t;code = 116
char = e;code = 101
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char = t;code = 116
char = o;code = 111
char = ;code = 32
char = p;code = 112
char = H;code = 72
char = ;code = 32
char = 2;code = 50
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char = 0;code = 48
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char = w;code = 119
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char = 8;code = 56
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char = a;code = 97
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char = ,;code = 44
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char = 1;code = 49
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char = ;code = 32
char = v;code = 118
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char = v;code = 118
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char = .;code = 46
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char = S;code = 83
char = A;code = 65
char = ;code = 32
char = a;code = 97
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char = S;code = 83
char = G;code = 71
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char = r;code = 114
char = e;code = 101
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char = t;code = 116
char = e;code = 101
char = c;code = 99
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char = e;code = 101
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char = b;code = 98
char = y;code = 121
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char = t;code = 116
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char = e;code = 101
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char = r;code = 114
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char = n;code = 110
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
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char = f;code = 102
char = l;code = 108
char = u;code = 117
char = o;code = 111
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char = e;code = 101
char = s;code = 115
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char = e;code = 101
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char = e;code = 101
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char = (;code = 40
char = e;code = 101
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char = c;code = 99
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char = t;code = 116
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = ;code = 32
char = 2;code = 50
char = 7;code = 55
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char = n;code = 110
char = m;code = 109
char = ,;code = 44
char = ;code = 32
char = e;code = 101
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char = i;code = 105
char = s;code = 115
char = s;code = 115
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char = o;code = 111
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char = ;code = 32
char = 3;code = 51
char = 4;code = 52
char = 0;code = 48
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char = m;code = 109
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char = ;code = 32
char = a;code = 97
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char = t;code = 116
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = ;code = 32
char = t;code = 116
char = i;code = 105
char = m;code = 109
char = e;code = 101
char = s;code = 115
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 7;code = 55
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = 1;code = 49
char = 3;code = 51
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char = m;code = 109
char = i;code = 105
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = p;code = 112
char = e;code = 101
char = c;code = 99
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char = i;code = 105
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = y;code = 121
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char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = o;code = 111
char = u;code = 117
char = t;code = 116
char = ;code = 32
char = i;code = 105
char = n;code = 110
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char = e;code = 101
char = r;code = 114
char = f;code = 102
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = -;code = 45
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = P;code = 80
char = a;code = 97
char = r;code = 114
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = e;code = 101
char = l;code = 108
char = ;code = 32
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char = ;code = 32
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char = w;code = 119
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char = i;code = 105
char = n;code = 110
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char = f;code = 102
char = i;code = 105
char = r;code = 114
char = m;code = 109
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = u;code = 117
char = l;code = 108
char = t;code = 116
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = R;code = 82
char = e;code = 101
char = c;code = 99
char = o;code = 111
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = s;code = 115
char = p;code = 112
char = i;code = 105
char = k;code = 107
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = s;code = 115
char = a;code = 97
char = m;code = 109
char = p;code = 112
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = (;code = 40
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char = .;code = 46
char = 1;code = 49
char = ;code = 32
char = m;code = 109
char = g;code = 103
char = /;code = 47
char = k;code = 107
char = g;code = 103
char = );code = 41
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = 8;code = 56
char = 0;code = 48
char = %;code = 37
char = ;code = 32
char = (;code = 40
char = c;code = 99
char = o;code = 111
char = e;code = 101
char = f;code = 102
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = i;code = 105
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = v;code = 118
char = a;code = 97
char = r;code = 114
char = i;code = 105
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = C;code = 67
char = .;code = 46
char = V;code = 86
char = .;code = 46
char = ;code = 32
char = =;code = 61
char = ;code = 32
char = 5;code = 53
char = %;code = 37
char = );code = 41
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = S;code = 83
char = A;code = 65
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = 6;code = 54
char = 2;code = 50
char = %;code = 37
char = ;code = 32
char = (;code = 40
char = C;code = 67
char = .;code = 46
char = V;code = 86
char = .;code = 46
char = ;code = 32
char = =;code = 61
char = ;code = 32
char = 6;code = 54
char = %;code = 37
char = );code = 41
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = S;code = 83
char = G;code = 71
char = .;code = 46
char = ;code = 32
char = C;code = 67
char = o;code = 111
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 2;code = 50
char = 7;code = 55
char = ;code = 32
char = m;code = 109
char = g;code = 103
char = /;code = 47
char = k;code = 107
char = g;code = 103
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = S;code = 83
char = A;code = 65
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 5;code = 53
char = ;code = 32
char = m;code = 109
char = g;code = 103
char = /;code = 47
char = k;code = 107
char = g;code = 103
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = S;code = 83
char = G;code = 71
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = a;code = 97
char = l;code = 108
char = y;code = 121
char = z;code = 122
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = m;code = 109
char = u;code = 117
char = s;code = 115
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = m;code = 109
char = e;code = 101
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = s;code = 115
char = w;code = 119
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = h;code = 104
char = i;code = 105
char = c;code = 99
char = h;code = 104
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = c;code = 99
char = e;code = 101
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = s;code = 115
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = m;code = 109
char = u;code = 117
char = s;code = 115
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = j;code = 106
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 2;code = 50
char = .;code = 46
char = 1;code = 49
char = ;code = 32
char = g;code = 103
char = ;code = 32
char = S;code = 83
char = A;code = 65
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 6;code = 54
char = ;code = 32
char = g;code = 103
char = ;code = 32
char = S;code = 83
char = G;code = 71
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = s;code = 115
char = l;code = 108
char = a;code = 97
char = u;code = 117
char = g;code = 103
char = h;code = 104
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = 2;code = 50
char = 4;code = 52
char = ;code = 32
char = h;code = 104
char = ;code = 32
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
result sql = A high-performance liquid chromatographic method is presented for the selective analysis of sulphanilamide (SA) and sulphaguanidine (SG) residues in animal tissues (muscle, liver), egg and milk at the 0.01-0.1 mg/kg level. A 25-g amount of the samples was extracted with 90 ml acetonitrile at pH 8.5 to which 2 ml saturated sodium carbonate had been added. After centrifugation, co-extracted water was separated by saturating the extract with sodium chloride and adding 50 ml dichloromethane. The residue of the dried and evaporated organic layer was partitioned between methanol, n-hexane and mobile phase (1 ml each). A 20-microliter volume of the aqueous layer was injected onto a Nucleosil 5 SA column [mobile phase 0.05 M sodium dihydrogenphosphate (adjusted to pH 2.0 with 85% orthophosphoric acid)-methanol, 85:15, v/v]. SA and SG were detected by their native fluorescence (excitation 275 nm, emission 340 nm) at retention times of 7 and 13 min, respectively, without interference of co-extractives. Parallel detection by ultraviolet absorption (275 nm) allowed on-line confirmation of the results. Recovery of spiked samples (0.1 mg/kg) was 80% (coefficient of variation, C.V. = 5%) for SA and 62% (C.V. = 6%) for SG. Concentrations of 0.27 mg/kg for SA and 0.05 mg/kg for SG were analyzed in muscle meat of a swine which received a single intramuscular injection of 2.1 g SA and 0.6 g SG and was slaughtered 24 h later.
Publication: J. Chromatogr. 1987 Dec 25 ;423:217-25.
Publication Date: 1987 Dec 25
Study Author(s): Petz, M;
Institution: Institut für Lebensmittelchemie, Universität Münster, B.R.D.
Shortcut link to this study:
Publication Date: 1987 Dec 25
Study Author(s): Petz, M;
Institution: Institut für Lebensmittelchemie, Universität Münster, B.R.D.
Shortcut link to this study:
A high-performance liquid chromatographic method is presented for the selective analysis of sulphanilamide (SA) and sulphaguanidine (SG) residues in animal tissues (muscle, liver), egg and milk at the 0.01-0.1 mg/kg level. A 25-g amount of the samples was extracted with 90 ml acetonitrile at pH 8.5 to which 2 ml saturated sodium carbonate had been added. After centrifugation, co-extracted water was separated by saturating the extract with sodium chloride and adding 50 ml dichloromethane. The residue of the dried and evaporated organic layer was partitioned between methanol, n-hexane and mobile phase (1 ml each). A 20-microliter volume of the aqueous layer was injected onto a Nucleosil 5 SA column [mobile phase 0.05 M sodium dihydrogenphosphate (adjusted to pH 2.0 with 85% orthophosphoric acid)-methanol, 85:15, v/v]. SA and SG were detected by their native fluorescence (excitation 275 nm, emission 340 nm) at retention times of 7 and 13 min, respectively, without interference of co-extractives. Parallel detection by ultraviolet absorption (275 nm) allowed on-line confirmation of the results. Recovery of spiked samples (0.1 mg/kg) was 80% (coefficient of variation, C.V. = 5%) for SA and 62% (C.V. = 6%) for SG. Concentrations of 0.27 mg/kg for SA and 0.05 mg/kg for SG were analyzed in muscle meat of a swine which received a single intramuscular injection of 2.1 g SA and 0.6 g SG and was slaughtered 24 h later.
PMID: 3443652
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