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char = n;code = 110
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char = l;code = 108
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char = n;code = 110
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char = l;code = 108
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char = p;code = 112
char = o;code = 111
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char = e;code = 101
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char = r;code = 114
char = e;code = 101
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = t;code = 116
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char = t;code = 116
char = u;code = 117
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = t;code = 116
char = a;code = 97
char = ;code = 32
char = 1;code = 49
char = -;code = 45
char = 4;code = 52
char = ;code = 32
char = g;code = 103
char = a;code = 97
char = l;code = 108
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = o;code = 111
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char = e;code = 101
char = -;code = 45
char = m;code = 109
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char = d;code = 100
char = i;code = 105
char = f;code = 102
char = i;code = 105
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = O;code = 79
char = -;code = 45
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = k;code = 107
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = N;code = 78
char = -;code = 45
char = a;code = 97
char = c;code = 99
char = e;code = 101
char = t;code = 116
char = y;code = 121
char = l;code = 108
char = g;code = 103
char = l;code = 108
char = u;code = 117
char = c;code = 99
char = o;code = 111
char = s;code = 115
char = a;code = 97
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = o;code = 111
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = i;code = 105
char = n;code = 110
char = s;code = 115
char = .;code = 46
result sql = Functional nuclear pores reconstituted with beta 1-4 galactose-modified O-linked N-acetylglucosamine glycoproteins.
Functional nuclear pores reconstituted with beta 1-4 galactose-modified O-linked N-acetylglucosamine glycoproteins.
char = N;code = 78
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char = l;code = 108
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char = p;code = 112
char = r;code = 114
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char = b;code = 98
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char = g;code = 103
char = l;code = 108
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char = G;code = 71
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char = r;code = 114
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char = t;code = 116
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = l;code = 108
char = t;code = 116
char = h;code = 104
char = o;code = 111
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char = g;code = 103
char = h;code = 104
char = ;code = 32
char = a;code = 97
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char = r;code = 114
char = o;code = 111
char = l;code = 108
char = e;code = 101
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char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = t;code = 116
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char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = o;code = 111
char = s;code = 115
char = y;code = 121
char = l;code = 108
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char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = n;code = 110
char = o;code = 111
char = t;code = 116
char = ;code = 32
char = e;code = 101
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char = t;code = 116
char = a;code = 97
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char = l;code = 108
char = i;code = 105
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char = d;code = 100
char = .;code = 46
char = ;code = 32
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char = u;code = 117
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char = e;code = 101
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char = g;code = 103
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char = e;code = 101
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char = c;code = 99
char = t;code = 116
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char = c;code = 99
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char = a;code = 97
char = b;code = 98
char = l;code = 108
char = e;code = 101
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char = m;code = 109
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char = g;code = 103
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char = c;code = 99
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char = i;code = 105
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char = a;code = 97
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char = ;code = 32
char = n;code = 110
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char = c;code = 99
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char = i;code = 105
char = ;code = 32
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char = t;code = 116
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char = e;code = 101
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char = ;code = 32
char = o;code = 111
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char = g;code = 103
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char = r;code = 114
char = m;code = 109
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char = a;code = 97
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char = g;code = 103
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char = M;code = 77
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char = o;code = 111
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char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = n;code = 110
char = u;code = 117
char = c;code = 99
char = l;code = 108
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char = e;code = 101
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char = r;code = 114
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char = i;code = 105
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char = g;code = 103
char = ;code = 32
char = i;code = 105
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char = h;code = 104
char = e;code = 101
char = a;code = 97
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char = ;code = 32
char = g;code = 103
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char = r;code = 114
char = m;code = 109
char = ;code = 32
char = a;code = 97
char = g;code = 103
char = g;code = 103
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char = u;code = 117
char = t;code = 116
char = i;code = 105
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char = ;code = 32
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char = i;code = 105
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char = n;code = 110
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char = t;code = 116
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char = N;code = 78
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char = ;code = 32
char = s;code = 115
char = y;code = 121
char = n;code = 110
char = t;code = 116
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char = e;code = 101
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char = i;code = 105
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result sql = Nuclear pore proteins bearing O-linked N-acetylglucosamine (GlcNAc) are involved in nuclear transport, although a role for their glycosylation is not established. Xenopus egg extracts capable of reforming functional nuclei in vitro yielded nuclei with impaired transport and reduced nuclear pore density when depleted of wheat germ agglutinin-binding proteins. Many of the nuclear pores remaining in wheat germ agglutinin-depleted nuclei showed a striking loss of internal structure. Nuclear transport and normal nuclear pore structure were restored by the addition of nuclear pore glycoproteins from rat liver or Xenopus eggs. Glycoproteins modified by the addition of galactose to O-linked GlcNAc were also competent for assembling normal nuclear pores and restoring nuclear transport. Aphidicolin-sensitive DNA synthesis was unaffected by the removal or modification of O-linked GlcNAc glycoproteins. These data argue against a requirement for a lectin-like recognition of O-linked GlcNAc glycoproteins in nuclear pore assembly, nuclear transport, or DNA synthesis.

Publication: J. Biol. Chem. 1994 Mar 25 ;269(12)::9289-97.
Publication Date: 1994 Mar 25
Study Author(s): Miller, M W;Hanover, J A;
Institution: Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Shortcut link to this study:
Nuclear pore proteins bearing O-linked N-acetylglucosamine (GlcNAc) are involved in nuclear transport, although a role for their glycosylation is not established. Xenopus egg extracts capable of reforming functional nuclei in vitro yielded nuclei with impaired transport and reduced nuclear pore density when depleted of wheat germ agglutinin-binding proteins. Many of the nuclear pores remaining in wheat germ agglutinin-depleted nuclei showed a striking loss of internal structure. Nuclear transport and normal nuclear pore structure were restored by the addition of nuclear pore glycoproteins from rat liver or Xenopus eggs. Glycoproteins modified by the addition of galactose to O-linked GlcNAc were also competent for assembling normal nuclear pores and restoring nuclear transport. Aphidicolin-sensitive DNA synthesis was unaffected by the removal or modification of O-linked GlcNAc glycoproteins. These data argue against a requirement for a lectin-like recognition of O-linked GlcNAc glycoproteins in nuclear pore assembly, nuclear transport, or DNA synthesis.
PMID: 8132666

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