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result sql = HPLC-fluorescence determination of unconjugated estrogens in pharmaceuticals.
HPLC-fluorescence determination of unconjugated estrogens in pharmaceuticals.
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result sql = A fluorimetric liquid chromatographic method (lambda(ex) = 280 nm; lambda(em) = 312 nm) was developed for measurements of unconjugated estrogens (estradiol and estriol) in pharmaceutical dosage forms using a reversed-phase column with water acetonitrile at different composition as mobile phase. The in vitro release profiles of three different estradiol transdermal therapeutic systems were determined through a medical-grade silicone rubber subdermal implant material membrane, using a modified Franz diffusion apparatus at 37 degrees C in presence of PEG 400. The HPLC method possesses advantages of rapidity, simplicity and accuracy.

Publication: J Pharm Biomed Anal 1998 Oct ;18(1-2)::187-92.
Publication Date: 1998 Oct
Study Author(s): Gatti, R;Gioia, M G;Di Pietra, A M;Cavrini, V;
Institution: Dipartimento di Scienze Farmaceutiche, Bologna, Italy. [email protected]
Shortcut link to this study:
A fluorimetric liquid chromatographic method (lambda(ex) = 280 nm; lambda(em) = 312 nm) was developed for measurements of unconjugated estrogens (estradiol and estriol) in pharmaceutical dosage forms using a reversed-phase column with water acetonitrile at different composition as mobile phase. The in vitro release profiles of three different estradiol transdermal therapeutic systems were determined through a medical-grade silicone rubber subdermal implant material membrane, using a modified Franz diffusion apparatus at 37 degrees C in presence of PEG 400. The HPLC method possesses advantages of rapidity, simplicity and accuracy.
PMID: 9863957

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